MetPAD/MetPLATE microbiology and toxicology 

 

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MetPAD™ and MetPLATE™ - heavy metal toxicity test kits

 

 

 

Refereed Publications

Dutton, R. J., G. Bitton, B. Koopman and O. Agami. 1990. Inhibition of b-galactosidase biosynthesis in Escherichia coli: Effect of alterations of the outer membrane permeability on sensitivity to environmental toxicants. Toxicity Assessment 5: 253-264.

Mazidji, C.N., B. Koopman and G. Bitton. 1992. Chelating resin versus ion- exchange resin for heavy metal removal in toxicity fractionation. Water Sci. Technol. 26: 189-196.

Mazidji, C.N., B. Koopman, G. Bitton and D. Neita. 1992. Distinction between heavy metal and organic toxicity using EDTA chelation and microbial assays. Environ. Toxicol. Water Qual. 7: 339-353.

Bitton, G., B. Koopman and O. Agami. 1992. MetPADTM: a bioassay for rapid assessment of heavy metal toxicity in wastewater. Water Environ. Res. 64: 834-836.

Bitton, G., M. Campbell and B. Koopman. 1992. MetPAD: A bioassay kit for the specific determination of heavy metal toxicity in sediments from hazardous waste sites. Environ. Toxicol. Water Qual. 7: 323-328.

de Vevey, E., G. Bitton, D. Rossel, L.D. Ramos, L. Munguia Guerrero and J. Tarradellas. 1993. Concentration and bioavailability of heavy metals insediments in Lake Yojoa (Honduras). Bull. Environ. Contam. Toxicol. 50: 253-259.

Bitton, G., K. Jung and B. Koopman. 1994. Evaluation of a microplate assay specific for heavy metal toxicity. Arch. Environ. Contam. Toxicol. 27: 25-28.

Ayoub, G., B. Koopman, G. Bitton and K. Riedesel. 1995. Heavy metal detoxification by trimercapto-s-triazine (TMT) as evaluated by a bacterial enzyme assay. Environ. Toxicol. Chem. 14 (2): 193-196.

Kong, I-C., G. Bitton, B. Koopman and K. Jung. 1995. Heavy Metal Toxicity Testing in Environmental Samples. Rev. Environ. Contam. Toxicol. 142: 119- 147.

Jung, K., G. Bitton and B. Koopman. 1996. Selective assay for heavy metal toxicity using a fluorogenic substrate. Environ. Toxicol. & Chem. 15: 711-714.

Rossel, D., J. Tarradellas, G. Bitton and J.L. Morel. 1996. Use of enzymes in soil ecotoxicology: A case for dehydrogenase and hydrolytic enzymes. In: Soil Ecotoxicology, J. Tarradellas, G. Bitton and D. Rossel, Eds., Lewis Publishers, Boca Raton, FL.

Bitton, G., E. Garland, I.C. Kong, J.L. Morel and B. Koopman. 1996. A Direct solid phase assay for heavy metal toxicity. I. Methodology. J. Soil Contam. 5: 385-394.

Boularbah, A., J.L. Morel, G. Bitton, and M. Mench. 1996. A Direct solid phase assay for heavy metal toxicity. II. Assessment of heavy metal Immobilization in soils and bioavailability to plants. J. Soil Contam. 5: 395-404..

Bitton, G. and J.L. Morel. 1998. Enzyme assays for the detection of heavy metal toxicity, In: Microscale Aquatic Toxicology: Advances, Techniques and Practice, P.G. Wells, K. Lee, and C. Blaise, Eds., CRC Press, Boca Raton, FL.
 

Boularbah, Ali, Gabriel Bitton, and J.L. Morel. 1999.  Assessment of metal contentoxicity of leachates from teapots.  Science for the Total Environment 227: 69-72.

Huang, Fenglei, Gabriel Bitton, and In-Chul Kong.  1999.  Determination of the Heavy Metal Binding Capacity of Aquatic Samples Using  MetPLATE: A Preliminary Study. Science for the Total Environment  234:139-145.

Boularbah, Ali, Gabriel Bitton, Jean Louis Morel, and Christophe Schwartz. 2000. Assessment of metal accumulation in plants using MetPAD, a toxicity test specific for heavy metal toxicity.  Environ. Toxicol. 15: 449-455

Dousset, Sylvie, Jean Louis Morel, Astrid Jacobson and Gabriel Bitton. 2001.           Copper binding capacity of root exudates of cultivated plants and associated            weeds.  Biol. Fertil. Soils 34: 230-234.

Bitton, G. 2002. Toxicity Testing in Wastewater Treatment Plants, Using Microorganisms, In:  Encyclopedia of Environmental Microbiology, G. Bitton,       Editor-In-Chief, John Wiley & Sons, New York

Ward, Marnie, Gabriel Bitton, Timothy Townsend and Matthew Booth.  2002. Toxicity of leachates from Florida municipal solid waste (MSW) landfills, using a battery of tests approach.  J. Environ. Toxicol.  17: 258-266.

Jang, Y.-C. and T.G. Townsend, M. Ward and G. Bitton. 2002. Leaching of arsenic, chromium, and copper in a contaminated soil at a  wood preserving site. Bull. Environ. Contam. Toxicol.  69: 808-816.

Kong, In-Chul and Gabriel Bitton. 2003. Correlation between toxicity and   metal fractions of contaminated soils in Korea.  Bull. Environ. Contam. Toxicol. 70: 557-565.

Stook, K., B. Dubey, M. Ward, T. Townsend, G. Bitton, and H. Solo-Gabriele. 2004. An evaluation of the heavy metal toxicity of pressure-treated wood   leachates with MetPLATE.  Bull. Environ. Contam. Toxicol. 73 (6): 987-994.

Gabriel Bitton, Marnie Ward, and Roi Dagan. 2005.  Determination of the heavy metal binding capacity (HMBC) of environmental samples.  In: pp. 215-231, Small-Scale Freshwater Environment Toxicity Test Methods, Volume 2, C. Blaise and J.F. Férard, editors, Kluwer Academic Publishers. N.Y.

Ward, Marnie, Gabriel Bitton and Timothy Townsend.  2005.  Heavy metal binding capacity (HMBC) of municipal solid waste landfill leachates. Chemosphere 60: 206-211.

Stook, Kristin, Thabet Tolaymat, Marnie Ward, Brajesh Dube, Timothy Townsend, Helena Solo-Gabriele, and Gabriel Bitton. 2005.  Relative leaching and aquatic toxicity of pressure-treated wood products using batch leaching tests. Environ. Sci. Technol. 39: 155-163.

Dubey, B., T. Townsend, G. Bitton, and H. Solo-Gabriele.  2005.  Comparison of temporal changes in metal leaching and aquatic toxicity from wood treated with CCA and alternative preservatives.  36th Annual Meeting of the Intern. Res. Group on Wood Protection, Bangalore, India, 24-28 April, 2005

Dagan, Roi, Gabriel Bitton, and Timothy Townsend. 2006. Metal Transport and Bioavailability in Soil Contaminated with CCA-Treated Wood Leachates. Soil and Sediment Contamination: An International Journal 15: 61-72

Boularbah, Ali, Christophe Schwartz, Gabriel Bitton and Jean Louis Morel. 2005. Heavy metal contamination from mining sites in South Morocco: 1.Use of a biotest to assess metal toxicity of tailings and soils. Chemosphere  63: 802-810.

Boularbah, Ali, Christophe Schwartz, Gabriel Bitton, Wafae Aboudrar, Ahmed Ouhammou, and Jean Louis Morel. 2005.  Heavy metal contamination from mining sites in south Morocco: 2. Assessment of metal accumulation and toxicity in plants.  Chemosphere 63: 811-817.

Dagan, R., and G. Bitton. 2005.  Toxicological Approach for assessing the heavy metal binding capacity of soils.  Soil and Sediment Contam. (In Press)

Dagan, R., B. Dubey, G. Bitton, and T. Townsend.  2007.  Aquatic toxicity of             leachates generated from electronic devices.  Arch. Environ. Contam. Toxicol.         (In Press).

Dubey, B., T. Townsend, H. Solo-Gabriele, and G. Bitton.  2007. Impact of surface water conditions on preservative leaching and aquatic toxicity from treated wood. Environ. Sci. & Technol. 41 (10): 3781-3786.

Dagan, R., B. Dubey, G. Bitton, and T. Townsend.  2007.  Aquatic toxicity of             leachates generated from electronic devices. Arch. Environ. Contam. Toxicol. 53: 168-173.

Feng, N. R. Dagan, and G. Bitton. 2007.  Toxicological Approach for assessing         the heavy metal binding capacity of soils.  Soil and Sediment Contam. 16: 1-8.

Feng, N., G. Bitton, P. Yeager, J.-C. Bonzongo, and  A. Boularbah. 2007. Heavy metal removal from soils using magnetic  separation: 1. Laboratory experiments. CLEAN Soil, Air, Water 35 (4): 362-369.

El Khalil, H., O. El Hamiani, G. Bitton, N. Ouazzani, and Ali Boularbah.  2008. Heavy metal contamination from mining sites in South Morocco: Monitoring metal content and toxicity of soil runoff and groundwater. Environ. Monit. & Assess. 136: 147-160. 

El Khalil, H., O. El Hamiani, G. Bitton, N. Ouazzani, and Ali Boularbah.  2007. Heavy metal contamination from mining sites in South Morocco: 3. Use of a biotest to assess metal toxicity of soil-runoff and groundwater. Environ. Monitoring Assess. (Submitted) 


Abstracts and Conference Proceedings

Jung, K., G. Bitton, and B. Koopman. 1994. A Rapid and Quantitative Assay for Heavy Metal Toxicity in Aquatic Samples. Presented at the 58th Annual Meeting of the Florida Academy of Sciences, March 24-26, 1994, Florida A&M University, Tallahassee, FL.

Huang, F., C. Kong, G. Bitton, and B. Koopman. 1994. Determination of Heavy Metal Binding Capacity (HMBC) in Natural and Waste Waters.Presented at the 58th Annual Meeting of the Florida Academy of Sciences,March 24-26, 1994, Florida A&M University, Tallahassee, FL.

Garland, E., I-C. Kong, G. Bitton, B. Koopman, and J.L. Morel. 1994. A New Microbial Test for the Specific Determination of Solids-Associated Heavy Metal Toxicity. Presented at the 58th Annual Meeting of the Florida Academy of Sciences, March 24-26, 1994, Florida A&M University, Tallahassee, FL.

Morel, J.L., E. Garland, G. Bitton and B. Koopman and I-C. Kong. 1994. A new microbial test for the specific determination of solids-associated heavy metal toxicity. Presented at the 1994 Luxembourg International Symposium on the Rehabilitation of Military Sites and Demilitarization of Explosive Ordnance. Kirchberg, Luxembourg,November 14-18, 1994.

Bitton, Gabriel. 1994. Development of rapid test kits for measuring heavy metal toxicity. Presented at the Workshop on Biosensors and Biological Techniques in Environmental Analysis, Ecole Superieure de Physique et de Chimie de Paris, Paris, FRANCE, September 12-14, 1994.

Jung, K., G. Bitton and B. Koopman. 1995. Fluorogenic substrate-based MetPLATE , a toxicity test specific for heavy metals. Proceeedings of the 1995 Spring Korean Environmental Engineering Conference, Inha University, Incheon, Korea, May 19-20, 1995.

Kuo, E. and G. Bitton. 1995. Surface modification of particles: Impact on removal of heavy metal toxicity. 59th Annual Meeting Florida Acad. Sci. , Tampa, FL., May 19-20,

Hosy, C., J-L. Morel and G. Bitton. 1995. Microbiological test to assess the bioavailability of metals to plants. Third Intern. Conference on the Biogeochemistry of Trace Elements, May 15-19, 1995, Versailles, France

Jung, K., G. Bitton, and B. Koopman. 1995. Short-term fluorogenic substrate-based microbial enzymatic toxicity test specific for heavy metals. Poster presented at Annual Conference of Air & Waste Management Association, San Antonio, TX, June 18-23, 1995.

Bitton, G. 1996. Microorganisms as tools for testing heavy metal toxicity in wastewater effluents and biosolids (abstract). Presented at the 8th. Inter. Congress of Bacteriology and Applied Microbiology Division. Jerusalem, Israel, Aug. 18-23, 1996.

Kong, I.-C., G. Bitton, and F. Huang. 1996. Use of MetPLATE for the determination of heavy metal binding capacity of environmental samples: Preliminary investigation, 18th IAWQ international conference, 23-28 June 1996, Singapore, p24 (P-EC02).

Bispo, A., M.J. Jourdain, M. Jauzein, C. Schwartz, C. Rothenflug, J.L. Morel, and G. Bitton. 1996. Assessment of Soil Toxicity with two microbiological tests. Presented at 4th European Conf. Ecotoxicology and Environmental Safety, Aug. 25-28, 1996, Metz, France.

Dousset, S., J.L. Morel, and G. Bitton. 1996. Metal Binding capacity of root exudates of cultivated plants and associated weeds. Presented at 2nd. Intern. Symp. On "Effect of Mineral-Organic-Microorganism Interactions on Soil and Freshwater Environments", Sept. 3-6, 1996, Nancy, France.

Boularbah, Ali, J.L. Morel, C. Schwartz, and G. Bitton. 1998. Use of MetPLATE for rapid assessment of heavy metal toxicity in soils, sludges, and sediments. Proceedings of the 16th World Congress of Soil Sciences, 20-26 August, 1998, Montpellier, France.
 

Boularbah, Ali, J.L. Morel, C. Schwartz, and G. Bitton. 1998.  Use of MetPLATE for rapid assessment of heavy metal toxicity in soils, sludges, and sediments.           Proceedings of the 16th World Congress of Soil Sciences, 20-26 August, 1998,         Montpellier, France.

Ward, M., G. Bitton, and T. Townsend. 1998.  Toxicological aspects of municipal       solid waste landfill leachates. Presented at the 1998 Southeastern Society of            Toxicology (SESOT) Conference "Interconnection between Ecological and                Human Toxicology", Oct. 22-23, 1998, University of Florida, Gainesville, FL.

Bitton, G., A. Boularbah, J.-L. Morel, and C. Schwartz. 1999. MetPLANT: A test for detecting metal-accumulating plants growing on contaminated soils. Presented at the 9th. International Symposium on Toxicity Assessment (ISTA 9), Pretoria, South Africa (Sept. 26-October 1, 1999).

Bitton, , I.C. Kong, J.L. Morel and F. Huang. 1999. Heavy metal binding capacity of environmental samples, using MetPAD/MetPLATE. Presented at the 9th. International Symposium on Toxicity Assessment (ISTA 9), Pretoria, South Africa (Sept. 26-October 1, 1999).

Gabriel Bitton, Ali Boularbah, A. Ouhammou, and Jean Louis Morel. 2001. Heavy Metal Contamination Resulting from an Abandoned Mine in the Vicinity  of Marrakech, Morocco, presented at the SETAC-Europe meeting, May 5-  12, 2001, Madrid, Spain. 

Gabriel Bitton, Ali Boularbah and Marnie Ward. 2001. MetSTICK:  Direct Screening of Heavy Metal Toxicity in Solids, Using Immobized Bacteria. Abstract 10th International  Symposium on  Toxicity Testing, August 2001, Quebec City, Canada

Kristin Stook, Marnie Ward, T. Townsend, G. Bitton, and M. Booth. 2001. Toxicity Evaluation of Leachates from Preserved Wood. Abstract 10th International  Symposium on  Toxicity Testing, August 2001, Quebec City, Canada

Ward, M., G. Bitton, T. Townsend, N. Denslow and M. Booth. 2001.                           Toxicological Aspects and Chemical Characterization of Leachates from                    Municipal Solid Waste Landfills in Florida.  Abstract 10th International                        Symposium on  Toxicity Testing, August 2001, Quebec City, Canada

Boularbah A., C. Schwartz, W. Aboudrar, A. Ouhammou, G. Bitton et J. L. Morel.    Biotest pour la détermination rapide de plantes  acumulatrices ou  hyperaccumulatrices de métaux. Communicatrion présentée au 2ème colloque du GMRE, Marrakech du 29 au 31  mai 2002

Boularbah, A., G. Bitton, C. Schwartz, W. Aboudrar and J. L. Morel. Assessment   of  Metal Accumulation in Plants Growing on Contaminated Soils. Presented at 7th   Conference on the Biogeochemistry of Trace-Elements, 2003, 15-19 June,             Upsalla, Sweden.

El khalil, Hicham, Ouafae El Hamiani, Gabriel Bitton, Naaila Ouazzani et  Ali Boularbah.  2005. Biotest Microbiologique MetPLATE pour évaluer la toxicité due aux métaux lourds dans les ressources en eau au voisinage de deux sites miniers situés dans la région de Marrakech.  In : Deuxième Congrès  Méditerranéen Ressources en Eau dans le Bassin Mediterraneen, Cadi Ayyad  University, Marrakech, November 14-17, 2005.

 
Other References on MetPAD/MetPLATE

Bispo, Antonio. 1998. Contribution a l'elaboration d'une methodologie pour evaluer les dangers et les risques lies aux materiaux solides contamines. These de Doctorat de l'Institut Polytechnique de Lorraine, INPL, Vandoeuvre les Nancy, FRANCE.

Hosy, C. 1994. Determination de la toxicite des metaux lourds dans les sols par le test microbiologique MetPLATE. Rapport de DEA "Sciences Agronomiques", ENSAIA-INPL, Vandoeuvres-les-Nancy, FRANCE.

Janssen, C, 1997. Alternative assays for routine toxicity assessments: A review. pp. 813-838. In: Ecotoxicology: Ecological Fundamentals, Chemical Exposure, and Biological Effects. G. Schuurmann and B. Markert, Eds. John Wiley and Sons, New York; Spektrum, Heidelberg, Germany.

Nelson, S.M. and R.A. Roline. 1998. Evaluation of the sensitivity of rapid toxicity tests relative to daphnid acute lethality tests. Bull. Environ. Contam. Toxicol. 60: 292-299.

Schwartz, C., A. Bispo, C. Rothenflug, M.J. Jourdain, M. Jauzein, and J.L. Morel. 1996. Assessment of soil toxicity with two microbiological tests. In: Ecotoxicology and Environmental Safety, SECOTOX Conference, Metz, FRANCE.

Kong, I.-C., C.-W. Lee, and Y.-T. Kwon. 1998. Heavy metal toxicity monitoring in sediments in Jinhae Bay, Korea. Bull. Environ. Contam. Toxicol. 61: 505-511.

Kwon, Y.-T., and C.W. Lee. 1998. Application of multiple ecological risk indices for the evaluation of heavy metal contamination in a coastal dredging area. The Science of the Total Environment 214: 203-210.

Brohon, B., and R. Gourdon. 2000. Influence of soil microbial activity level on the determination of contaminated soil toxicity using Lumistox and MetPlate bioassays. Soil Biol. Biochem. 32 (6): 853-857.

Babut, M., Perrodin, Y., Bray, M., Clément, B., Delolme, C., Devaux, A., Durrieu, C., Garric, J., Vollat, B., Becart, D. and C. Charrier (2002). Évaluation des risques écologiques causés par des matériaux de dragage : proposition d'une approche adaptée aux dépôts en gravière en eau. Rev. Sci. Eau 15 (3): 615-639.

Schultz, E., Joutti, A., Räisänen, M. L., Lintinen, P., Martikainen, E., Lehto, O. 2004. Extractability of metals and ecotoxicity of soils from two old wood impregnation sites in Finland. Science for the Total Environment.  326 (No. 1/3): 71-84

Blumenstain, P.E. 2004. Comparison of enzymatic bioassay to conventional aquatic toxicity testing in a mining influenced stream. 2004.  Denver Annual Meeting (November 7–10, 2004).

Wildeman, T.R., J. Ranville, E. Blumenstein, K.S. Smith and L. Choate. 2005. Advances in simple methods of assessing the toxicity of mine wastes. 2005 Salt Lake City Annual Meeting (Oct. 16-19, 2005).

Blumenstein, Eric,  James F. Ranville, Ladonna Choate, Philippe Ross, and Thomas Wildeman 2006. Evaluating the Effects of Water Chemistry Variation on an Enzyme Bioassay, MetPlate, Used to Screen for Metals Contamination in Mining Impacted Soils and Waters. 18th World Congress of Soil Science. July 9-15, 2006, Philadelphia, PA.

Jeanne GARRIC, Marc BRAY, Bernard VOLLAT, Bernard CLEMENT, Alain DEVAUX, Cécile DELOLME, Claude DURRIEU, Jean-Philippe BEDELL. 2001. Evaluation écotoxicologique de sédiments contaminés ou de matériaux de dragage. Report  http://www.lyon.cemagref.fr/bea/tox/rapports_pdf/RapfinalEssais.PDF

 

POWERPOINT PRESENTATIONS

http://lqma.ifas.ufl.edu/SEED/SOS6262/Lecture%20notes/MetPLATE_2006_Soil%20Sci.pdf

http://pubs.usgs.gov/of/2003/ofr-03-210/II_Bioaccessibility.pdf

http://pubs.usgs.gov/of/2003/ofr-03-210/Section508/II_Bioaccessibility-508.pdf

 

 

 

Updated on Feb 15, 2008

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